Screening crystallisation conditions using fluorescence correlation spectroscopy

Publication Type:

Journal Article

Authors:

Schmauder, R.; Schmidt, T.; Abrahams, J.P.; Kuil, M.E.

Source:

Acta Crystallographica Section D-Biological Crystallography, Volume 58, p.1536-1541 (2002)

ISBN:

0907-4449

DOI Name (links to online publication)

10.1107/S0907444902014373

Keywords:

diffusion; lysozyme; fluorescence correlation spectroscopy; crystallisation conditions; high magnetic-field; protein crystallization; self-diffusion; globular-proteins; lysozyme chloride; crystal-growth; coefficients; aggregation; nacl; ions

Abstract:

We investigate the potential of fluorescence correlation spectroscopy (FCS) in screening for crystallisation conditions. Solutions that nucleate protein crystals must have different interactions than solutions that do not give rise to crystals. Due to these different interactions the average mean squared displacement of the individual proteins changes. By monitoring protein self-diffusion, we can distinguish crystallising from non-crystallising solutions. The method introduced can be applied at extremely low concentrations in femtoliter volumes as an early diagnostic for molecular association. Based on our preliminary findings FCS has the potential to become a routine screening method for crystallography.